Synechococcus elongatus strains were derived from S. elongatus PCC 7942 (Syn7942). This strain was obtained from Prof. Susan Golden’s lab (University of California San Diego, UCSD). We used S. cerevisiae ρ + NB97 (MATa leu2-3,112 lys2 ura3-52 his3ΔHindIII arg8Δ::URA3 [cox2-60]) as a host for S. elongatus endosymbionts. The S. cerevisiae-cox2-60 strain was obtained from the Schultz lab (Scripps Research).
S. elongatus cells were grown in sterile Erlenmeyer flasks containing liquid BG-11 medium. These cultures were shaken aerobically at 37 °C and 250 rpm under 3000 lux. Yeast cells were shaken aerobically at 30 °C and 250 rpm in YPD medium (1% yeast extract, 2% peptone, 2% glucose) containing 50 mg/L carbenicillin.
List of all the fusion selection media:
Selection medium I: 1% yeast extract, 2 % peptone, 3 % glycerol, 0.1 % glucose, 1 M sorbitol, 2 % agar, and 1X BG-11 salts.
Selection
